Comparison of affinity tags for protein purification
id:fortuneorigin さんのところから。なんとなくお世話になりそうな論文なのでメモだけトランスファ。
HIS, CBP, CYD (covalent yet dissociable NorpD peptide), Strep II, FLAG, HPC (heavy chain of protein C) peptide tags, and the GST and MBP protein fusion tag systems の比較を行っている。Abstract だけ流し読みしてまとめておく。
Tag | Purity | Resin |
---|---|---|
HIS | Moderate in E. coli, poor in Drosophila, HeLa, yeast | Inexpensive, high capacity |
CBP | Moderate in E. coli, Drosophila better in HeLa | Not described in abstract |
Flag and HPC | Highest purity | Expensive and low capacity |
StrepII | Excellent | Moderate cost and good yield |
著者らのおすすめはStrep IIらしい。今度機会があったら試してみよ。